The following Acapella Script (RWC_Co-localization.script) is a modification of the co-localization script by T.J.Collins (MacBioPhotonics, McMaster University, Hamilton, Canada) to include Rank Weighted Co-localization coefficients for use with Columbus Image Data Storage and Analysis System (PerkinElmer).

     Download the Acapella Script here.

     Author: Vasanth R Singan (VRSingan @ lbl.gov)

NOTE

• The scripts works on a 3 channel image as input.
• User can decide the two channels on which RWC1 and RWC2 will be calculated.

Please cite our work:

Dual channel rank-based intensity weighting for quantitative co-localization of microscopy images

VR Singan, TR Jones, KM Curran, JC Simpson

BMC bioinformatics 12 (1), 407

Quantitative image analysis approaches for probing rab gtpase localization and function in mammalian cells

VR Singan, K Handzic, JC Simpson

Biochemical Society Transactions 40 (6), 1389

Implementation of the Rank-Weighted Co-localization (RWC) algorithm in multiple image analysis platforms for quantitative analysis of microscopy images

VR Singan, JC Simpson

Source Code for Biology and Medicine 11 (2)