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About Us

Page history last edited by Vasanth Singan 13 years, 1 month ago

About us            Lecture Notes          Protocols

 

Welcome to the Simpson Lab at University College Dublin!

 

The internal organelles of mammalian cells provide the possibility of keeping proteins with different functions in separate locations. This is achieved through membrane traffic, a process that uses vesicular and tubular membrane carriers to transport proteins between compartments in both the secretory and endocytic systems. Our lab is interested in how these pathways are organised, and what is the protein machinery that regulates the timing and specificity of trafficking steps.

 

The lab is primarily using high-throughput light microscopy approaches to achieve this. In particular we are employing recombination cloning, GFP-tagging, and RNA interference to identify the players involved and assess their relevance to particular trafficking steps. Key techniques include:

  • High-throughput GFP-tagging and localisation of novel proteins
  • RNA interference
  • Reverse transfection of cell arrays
  • High content screening (HCS) and cell-based assays
  • Advanced light microscopy approaches

 

People

 

Contact Us

          UCD Science Education and Research Centre - West

          University College Dublin

          Belfield, Dublin 4, Ireland

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