Welcome to the Simpson Lab at University College Dublin!
The internal organelles of mammalian cells provide the possibility of keeping proteins with different functions in separate locations. This is achieved through membrane traffic, a process that uses vesicular and tubular membrane carriers to transport proteins between compartments in both the secretory and endocytic systems. Our lab is interested in how these pathways are organised, and what is the protein machinery that regulates the timing and specificity of trafficking steps.
The lab is primarily using high-throughput light microscopy approaches to achieve this. In particular we are employing recombination cloning, GFP-tagging, and RNA interference to identify the players involved and assess their relevance to particular trafficking steps. Key techniques include:
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High-throughput GFP-tagging and localisation of novel proteins
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RNA interference
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Reverse transfection of cell arrays
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High content screening (HCS) and cell-based assays
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Advanced light microscopy approaches
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Contact Us
UCD Science Education and Research Centre - West
University College Dublin
Belfield, Dublin 4, Ireland
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